Comparison of real-time PCR, nested PCR, and galactomannan antigen detection by enzyme-linked immunosorbent assay in sera for diagnosis of invasive aspergillosis
Publication date: Available online 16 August 2017
Source:Journal de Mycologie Médicale / Journal of Medical Mycology
Author(s): S. Hedhili, N. Baccouchi, H. Souid, B. Jemli, S. Gargouri
Conventional methods for diagnosis of invasive aspergillosis (IA) lack sensitivity and specificity. Serological methods still have many cases of cross-reactivity. However, molecular techniques seem to arise as a rapid approach, specific and direct that could be used in the diagnosis of IA. In this study, we analyzed 88 serum samples from patients of having IA using GM-ELISA test, nested PCR with primers for...